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A novel high throughput microfluidic cell transit analyzer using air liquid interface as localized pressure sensor

TitleA novel high throughput microfluidic cell transit analyzer using air liquid interface as localized pressure sensor
Publication TypeConference Paper
Year of Publication2018
AuthorsKotesa, RS, Joshi, KR, Ananthasuresh, GK, Sen, P
Conference NameMicro Electro Mechanical Systems (MEMS), 2018 IEEE
PublisherIEEE
KeywordsHydrodynamics, microfluidics, Oscillators, Pressure measurement, Sociology, Statistics, Time measurement
Abstract

We report a novel microfluidic platform that measures pressure drop during the passage of a single cell through a mechanical constriction for mechano-phenotyping of cell populations. It uses a precisely positioned Air-Liquid Interface as a Sensor (ALIaS) near the outlet of the constriction. When cells pass through the constriction they momentarily plug the inlet resulting in a drop in the hydrodynamic pressure at the outlet of the constriction. This change in pressure leads to motion of the air-liquid interface which can be correlated to entry and transit time of the cell passage. ALIaS facilitates accurate measurements of cell entry and transit time at high throughout (>200 cells/s). The platform can reliably quantify the variation in deformability in cell populations upon treatment with Glutaraldehyde. The system presents a potential for mechanobiological studies and can be made portable through electrical measurements of interface oscillations.

DOI10.1109/MEMSYS.2018.8346501