Ti3C2-MXene decorated with nanostructured silver as a dual-energy acceptor for the fluorometric Neuron Specific Enolase detection

Nanohybrids of two-dimensional (2D) layered materials have shown fascinating prospects towards the fabrication of highly efficient fluorescent immunosensor. In this context, a nanohybrid of ultrathin Ti₃C₂-MXene nanosheets and silver nanoparticles (Ag@Ti₃C₂-MXene) has been reported as a dual-energy acceptor for ultrahigh fluorescence quenching of protein-functionalized graphene quantum dots (anti-NSE/amino-GQDs). The Ti₃C₂-MXene nanosheets are decorated with silver nanoparticles (AgNPs) to obsolete the agglomeration and restacking through a one-pot direct reduction method wherein the 2D Ti₃C₂-MXene nanosheets acted both as a reducing agent and support matrix for AgNPs. The as-prepared nanohybrid is characterized by various techniques to analyze the optical, structural, compositional, and morphological parameters. The quenching efficiency and energy transfer capability between the anti-NSE/amino-GQDs (donor) and Ag@Ti₃C₂-MXene (acceptor) have been explored through steady state and time-resolved spectroscopic studies. Interestingly, the Ag@Ti₃C₂-MXene nanohybrid exhibits better quenching and energy transfer efficiencies in contrast to bare Ti₃C₂-MXene, AgNPs and previously reported AuNPs. Based on optimized donor-acceptor pair, a fluorescent turn-on biosensing system is constructed that revealed improved biosensing characteristics compared to Ti₃C₂-MXene, graphene and AuNPs for the detection of neuron-specific enolase (NSE), including higher sensitivity (∼771 mL ng⁻¹), broader linear detection range (0.0001–1500 ng mL⁻¹), better LOD (0.05 pg mL⁻¹), and faster response time (12 min). Besides, remarkable biosensing capability has been observed in serum samples, with fluorescence recovery of ∼98%.